Prokaryotic expression of ropporin and generation of its rabbit polyclonal antisera
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چکیده
منابع مشابه
Prokaryotic Expression of NSP5 Gene of Human Group B Rotavirus and Generation of its Polyclonal Antibody
A pair of primers were designed according to the nsp5 gene of human group B rotavirus strain WH-1, and the nsp5 gene was amplified by PCR and subcloned into the expression vector pGEX-KG. After induced with IPTG, the NSP5 was expressed as a GST fusion protein in the soluble form. Then the protein was purified and immunized mice to obtain specific antiserum. At last, polyclonal antibody was anal...
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پس از بررسی هر کدام از فاکتورهای نوع صنعت, نوع ضمانت نامه, نرخ بهره , نرخ تورم, ریسک اعتباری کشورها, کارمزد, ریکاوری, gdp, پوشش و وثیقه بر ریسک اعتباری صندوق ضمانت صادرات ایران مشخص گردید که همه فاکتورها به استثنای ریسک اعتباری کشورها و کارمزد بقیه فاکتورها رابطه معناداری با ریسک اعتباری دارند در ضمن نرخ بهره , نرخ تورم, ریکاوری, و نوع صنعت و ریسک کشورها اثر عکس روی ریسک اعتباری داردو پوشش, وثی...
15 صفحه اولMonoclonal and polyclonal antisera
The extensive application of the hybridoma technology for tissue phenotyping has demonstrated in the last decade that a number of heterodimeric molecules consisting of noncovalently associated α and β subunits (Hynes, 1992), named integrins, play a major role in cell-to-cell and cellto-interstitium interactions as well as in cell trafficking (Hemler, 1990; Albelda and Buck, 1990). The structura...
متن کاملProkaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein
Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...
متن کاملProkaryotic expression of chicken infectious anemia apoptin protein and characterization of its polyclonal antibodies.
In the present study recombinant VP3 (rVP3) was expressed in E. coli BL21 (DE3) (pLysS) and its polyclonal antibodies were characterized. SDS-PAGE analysis revealed that the expression of recombinant protein was maximum when induced with 1.5 mM IPTG for 6 h at 37 degrees C. The 6xHis-tagged fusion protein was purified on Ni-NTA and confirmed by Western blot using CAV specific antiserum. Rabbits...
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ژورنال
عنوان ژورنال: World Chinese Journal of Digestology
سال: 2009
ISSN: 2219-2859
DOI: 10.11569/wcjd.v17.i32.3342